Author

Ratih Dewi Hastuti

Observed names: Hastuti, Ratih Dewi, K., R. D. Hastuti, R.D. Hastuti, RATIH DEWI HASTUTI, Ratih D. Hastuti, Ratih Dwi Hastuti
OpenAlex ID: A5032464002
Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants inoculated with only Streptomyces spp. Plants inoculated with Xoo and sprayed with a chemical bactericide, and plants inoculated with only Xoo served as positive controls, whereas plants not inoculated with either Streptomyces spp. or Xoo were used as negative controls. The results showed that the effect of endophytic Streptomyces spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P > 0.05). However, plants inoculated with endophytic Streptomyces spp. were significantly taller and produced higher tiller number than control plants (P < 0.05). Streptomyces spp. isolate AB131-1 gave the highest plant height. In vitro studies on biocontrol mechanisms of selected Streptomyces spp. isolates showed that isolate LBR02 gave the highest inhibition activity on Xoo growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic Streptomyces spp. from different species. These studies found that the paddy plant which was inoculated with endophytic Streptomyces spp. AB131-1 and infected by Xoo could increase the height of plant and number of tillers.
2013 · RATIH DEWI HASTUTI, YULIN LESTARI, et al. · HAYATI Journal of Biosciences · 82
2009 · Puspita Lisdiyanti, Misa Otoguro, et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 27
2012 · K. · American Journal of Agricultural and Biological Sciences · 14
2009 · M. Otoguro, S. Ratnakomala, et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 11
2016 · Shanti Ratnakomala, Puspita Lisdiyanti, et al. · BIOTROPIA · 10
1996 · R.D.M. Simanungkalit, R.J. Roughley, et al. · Soil Biology and Biochemistry · 9
2012 · YANTYATI WIDYASTUTI, PUSPITA LISDIYANTI, et al. · Microbiology Indonesia · 5
2016 · Radite Tistama, Cici Indriani Dalimunthe, et al. · Jurnal Penelitian Karet · 3
2010 · Puspita Lisdiyanti, Misa Otoguro, et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 2
2018 · Shanti Ratnakomala, Puspita Lisdiyanti, et al.
2012 · Hastuti, Ratih Dewi · IPB Scientific Repository (Bogor Agricultural University.)
Works count: 20
Citation count: 195
H-index: 7
I10-index: 5
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