Author

Willem P.C. Stemmer

Observed names: Stemmer, Willem P.C., W P Stemmer, W. P. C. Stemmer, WIllem P.C. Stemmer, Willem P C Stemmer, Willem P. C. Stemmer, Willem P.C Stemmer, Willem PC Stemmer, Willem Pim C Stemmer, Willem Stemmer

Observed institutions: Maxygen (United States), National Institutes of Health, Codexis (United States), Royal Tropical Institute, Amunix (United States), Stanford Medicine, Affymax (United States), Stanford University

OpenAlex ID: A5061394106

Rapid evolution of a protein in vitro by DNA shuffling

1994 · Willem P. C. Stemmer · Nature · 1,956

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

1994 · W P Stemmer · Proceedings of the National Academy of Sciences · 1,300

Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

1996 · Andreas Crameri, Erik A. Whitehorn, et al. · Nature Biotechnology · 1,201

DNA shuffling of a family of genes from diverse species accelerates directed evolution

1998 · Andreas Crameri, Sun-Ai Raillard, et al. · Nature · 834

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

1995 · Willem P.C. Stemmer, Andreas Crameri, et al. · Gene · 734

Genome shuffling leads to rapid phenotypic improvement in bacteria

2002 · Ying-Xin Zhang, Kim Perry, et al. · Nature · 563

A recombinant polypeptide extends the in vivo half-life of peptides and proteins in a tunable manner

2009 · Volker Schellenberger, Chia-wei Wang, et al. · Nature Biotechnology · 467

Genome shuffling of Lactobacillus for improved acid tolerance

2002 · Ranjan Patnaik, Susan Louie, et al. · Nature Biotechnology · 377

Directed evolution of proteins by exon shuffling

2001 · Joost A. Kolkman, Willem P.C. Stemmer · Nature Biotechnology · 274

Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening

1997 · Ji-Hu Zhang, Glenn Dawes, et al. · Proceedings of the National Academy of Sciences · 273

Multivalent avimer proteins evolved by exon shuffling of a family of human receptor domains

2005 · Joshua Silverman, Qiang Lu, et al. · Nature Biotechnology · 244

Predicting the emergence of antibiotic resistance by directed evolution and structural analysis.

2001 · M. Cecilia Orencia, Jun S. Yoon, et al. · Nature Structural Biology · 236

DNA shuffling of subgenomic sequences of subtilisin

1999 · Jon E. Ness, Mark Welch, et al. · Nature Biotechnology · 226

Directed Evolution and Biocatalysis

2001 · Keith A. Powell, Sandra W. Ramer, et al. · Angewandte Chemie International Edition · 225

Molecular evolution of an arsenate detoxification pathway by DNA shuffling

1997 · Andreas Crameri, Glenn Dawes, et al. · Nature Biotechnology · 178

Applications of DNA shuffling to pharmaceuticals and vaccines

1997 · Phillip A Patten, Russell J Howard, et al. · Current Opinion in Biotechnology · 154

Evolution of a cytokine using DNA family shuffling

1999 · Chia-Chun J. Chang, Teddy T. Chen, et al. · Nature Biotechnology · 128

Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

1999 · Fred C. Christians, Leonardo Scapozza, et al. · Nature Biotechnology · 126

Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes

2001 · SunAi Raillard, Anke Krebber, et al. · Chemistry & Biology · 114

PDF

Improving the Catalytic Activity of a Thermophilic Enzyme at Low Temperatures

2000 · Astrid Merz, Muh-ching Yee, et al. · Biochemistry · 110

Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis.

1992 · W P Stemmer, S K Morris · PubMed · 104

Molecular breeding of viruses

2000 · Nay-Wei Soong, Laurel Nomura, et al. · Nature Genetics · 103

Construction and evolution of antibody–phage libraries by DMA shuffling

1996 · Andreas Crameri, Steve Cwirla, et al. · Nature Medicine · 98

Breeding of retroviruses by DNA shuffling for improved stability and processing yields

2000 · Sharon K. Powell, Michele A. Kaloss, et al. · Nature Biotechnology · 84

A Novel Long-Acting Human Growth Hormone Fusion Protein (VRS-317): Enhanced In Vivo Potency and Half-Life

2012 · Jeffrey L. Cleland, Nathan C. Geething, et al. · Journal of Pharmaceutical Sciences · 77

PDF

Works count: 63

Citation count: 10,990

H-index: 36

I10-index: 45