Directed evolution
Concept
Site-directed mutagenesis by overlap extension using the polymerase chain reaction
1989 · Steffan N. Ho, Henry D. Hunt, et al. · Gene
Rapid evolution of a protein in vitro by DNA shuffling
1994 · Willem P.C. Stemmer · Nature
Yeast surface display for screening combinatorial polypeptide libraries
1997 · Eric T. Boder, K. Dane Wittrup · Nature Biotechnology