Effect of acetic acid on the activation of G-protein-coupled receptor GPR43 in skeletal muscle cells and on the skeletal muscle function
Description: GPR43 functioning involves the suppression of lipid accumulation and maintaining body energy homeostasis, and is activated by acetic acid or propionic acid. It was hypothesized that acetic acid would stimulate GPR43 in skeletal muscle cells and has function in modulating skeletal muscle function. The objective of the current study was to clarify the expression of GPR43 and the effect of acetic acid on the activation of GPR43 in the skeletal muscle cells. The GPR43 expression, observed in the differentiated myotube cells, was increased upon acetic acid treatment. Acetic acid induced the intracellular calcium influx and modulated genes expression associated with slow-twitch fibers in the cells and these effects were significantly inhibited by the GPR43-specific siRNA treatment. It was concluded that acetic acid plays a role in the activation of GPR43 and involves the proliferation of slow-twitch fibers in L6 skeletal muscles through the calcium-signaling pathway.
Amount: ¥4,420,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Hiromi Yamashita
Institution: Okayama Prefectural University
Funded outputs count: 5
Funder award ID: 18K05476
OpenAlex ID: G1246813828