Analysis on the role of SLITRK6 in congenital CMV infection
Description: Cytomegalovirus (CMV) infection in pregnant women causes multiple organ damages in fetuses such as sensorineural hearing loss and neurodevelopmental impairment. To understand the molecular mechanism of CMV neuropathogenesis, we previously identified SLITRK6, a known causative gene for hereditary hearing loss, as a potentially important gene involved in CMV pathogenesis. To understand the relevance of SLITRK6 to CMV neuropathogenesis, we established human neural cells in which the expression of SLITRK6 was experimentally downregulated by shRNA or upregulated by SLITRK6 expression plasmid. Using the cells, we performed microarray analysis and identified cellular genes which may be regulated by SLITRK6. Further, murine cytomegalovirus (MCMV) was experimentally infected to mouse neural cells to analyze the effect of MCMV infection on mouse Slitrk6 expression. These experimental systems will be useful to understand the mechanism of CMV-induced neural damages through SLITRK6.
Amount: ¥4,550,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2017-04-01
End date: 2020-03-31
Investigator: Shigeyoshi Fujiwara
Institution: National Center for Child Health and Development
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Funded outputs count: 4
Funder award ID: 17K10068
OpenAlex ID: G1306297612