Functional analyses of tisuue engneered thyroid gland fabricated with human iPS cells derived-thyrocytes
Description: The replacement of regenerated thyroid follicular cells (TFCs) is a promising therapeutic strategy for patients with hypothyroidism. Differentiation of human induced pluripotent stem cells (iPSCs) to TFCs was induced using a bioreactor developed with clinical application and we analyzed these cells genetically and histologically. At first, suspension culture with Activin A treatment produced definitive endodermal cells that expressed Sox17 and FoxA2 in the RT-PCR and flow cytometric analysis. Further treatment with thyroid-stimulating hormone (TSH) induced TFCs expressing thyroid transcription factors Pax8, Nkx2-1 and various types of thyroid proteins including TSH receptor, sodium-iodide symporter, thyroglobulin and thyroid peroxidase. Interestingly, differentiated cells secreted free thyroxine in vitro. These results indicate successful differentiation of human iPSCs to functional TFCs that may enable us to fabricate thyroid tissues for regenerative medicine and disease models.
Amount: ¥3,900,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (B)
Start date: 2016-04-01
End date: 2018-03-31
Investigator: Ayumi Arauchi
Institution: Tokyo Women's Medical University
Funded outputs count: 2
Funder award ID: 16K19561
OpenAlex ID: G193391268