Establishment of three-dimensional laminated lymphatic network autografts based on adipose tissue-derived stem cells (ASCs)
Description: Artificial three-dimensional vascular network tissues based on human adipose tissue-derived mesenchymal stem cells (hASCs) were constructed due to the aim of regenerative medicine for secondary lymphedema. Compared to the previous fibroblast-based cases, the promotion of vascular and lymphatic network formation was observed, and the three-dimensional hASC-based tissue was found to be more effective than the conventional fibroblast-based case in the formation of vascular network. The results suggested that the enhancement of expression of angiogenesis-related factors by hASCs was involved. Transplantation of vascular network tissue resulted in good engraftment with pericyte or smooth muscle-like differentiation of hASCs. On the other hand, transplantation of lymphatic network tissue showed regression of vascular vessels, but the results in the mouse model of lymphatic flow regeneration suggested anastomosis between deep and superficial lymphatic vessels and new channel formation.
Amount: ¥4,680,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2017-04-01
End date: 2022-03-31
Investigator: Yoshiya Asano
Institution: Hirosaki University
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Funded outputs count: 4
Funder award ID: 17K08506
OpenAlex ID: G1979126000