Kinetic analysis of gene expression regulation using visualization and epigenomic manipulation of specific genomic regions
Description: We investigated the dynamics of chromatin structural changes and transcriptional activation in live-cell imaging by combining post-translational modification specific probes against histone and RNA Polymerase 2 (RNAPol2) with CRISPR/Cas9-based visualization of specific genomic regions and manipulation of the epigenome. The histone acetylation including H3K27ac preceded and was independent of activation of RNAPol2. The chromatin structure changed and the mobility correlated with histone modifications and RNAPol2 states.
Amount: ¥4,290,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (B)
Start date: 2017-04-01
End date: 2021-03-31
Investigator: Tetsuya Handa
Institution: Tokyo Institute of Technology
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 11
Funder award ID: 17K17719
OpenAlex ID: G2071071611