Elucidation of the role of biological defense mechanisms by the intracellular proteolysis systems in damages of the inner ear
Description: To investigate the role of the intracellular proteolysis systems in damages in the inner ear, we analyzed the roles of the ubiquitin-proteasome system and the autophagy-lysosome system.To investigate the role of the autophagy-lysosome system in the inner ear, we generated mice deficient in a gene essential for autophagy (Atg5) in hair cells in the inner ear. Deletion of Atg5 resulted in hair cell degeneration and profound congenital hearing loss. To investigate the role of the ubiquitin-proteasome system in the inner ear, we analyzed the effect of proteasome inhibitor (MG-132) in cultured inner ear cells under oxidative damage. The low concentration of proteasome inhibitor had some protective effect, but it had toxic effect in the higher concentration.
Amount: ¥18,070,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (B)
Start date: 2013-04-01
End date: 2017-03-31
Investigator: Shinichi Iwasaki (lead)
Institution: The University of Tokyo
Funded outputs count: 8
Funder award ID: 25293347
OpenAlex ID: G251147489