Role of paraventricular thalamic nucleus in bipolar disorder
Description: In bipolar patients and controls, paraventricular thalamic nucleus (PVT) were identified by calretinin staining. Double staining with anti-cytochrome c oxidase (COX) and anti-succinate dehydrogenase (SDH) antibodies was used to identify cells with mitochondrial dysfunction and anti-8-OHdG antibody immunostaining was used to evaluate DNA oxidation. Cells with mitochondrial dysfunction were identified in the PVT. We attempted to create a reporter mouse that expresses Cre specifically in the PVT by dividing Cre into two parts and expressing them as a fusion protein via a 2A sequence downstream of Gbx2 and Maob, and then aggregating them by drug administration. However, Cre activity was not obtained, and adeno-associated viruses were used in subsequent experiments.
Amount: ¥40,430,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (A)
Start date: 2017-04-01
End date: 2022-03-31
Investigator: TADAFUMI KATO
Institution: Juntendo University(2020-2021)Institute of Physical and Chemical Research(2017-2019)
Funded outputs count: 16
Funder award ID: 17H01573
OpenAlex ID: G2699744711