Study of bone regeneration therapy using Human Umbilical Cord PeriVascular cells differentiated and induced in serum-free medium
Description: We focused on Human umbilical cord perivascular cells (HUCPVCs) as a new cellular source.The objective of this study is to investigate bone differentiation induction of HUCPVCs based on the collection term of bone marrow cell’s condition medium.BM-CM was divided into three according to the collection time.（Proliferate, Confluent, Calcification）HUCPVCs were cultured with each period of BM-CM, and the state of differentiation was evaluated in RT-PCR, ALP staining and Alizarin staining.Confluent and Calcification groups for 2 weeks showed more expression of Runx 2 than other group. HUCPVCs which were cultured with Confluent BM-CM for 2 weeks showed positive staining with ALP staining and Alizarin staining than other groups. In the conventional method, HUCPVCs is required to culture for 3 weeks before calcification. The results suggested that HUCPVCs which are cultured using confluent BM - CM show calcification tendency in 2 weeks.
Amount: ¥3,380,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (B)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: Sohtaro Kajiyama
Institution: Tsurumi University
Funded outputs count: 0
Funder award ID: 16K20547
OpenAlex ID: G2870396107