Development of single cell-based technologies for iPSC-neurons from patients with schizophrenia
Description: In this study, we have developed a series of recombinant lentiviruses expressing a fluorescent Venus protein under the control of promoters from various cell-type-specific marker genes for constructing cell-type specific labeling systems for iPSC-differentiated neurons from patients with psychiatric disorders. Using lentiviruses, we have successfully separated cell-type specific neurons from iPSC-differentiated neurons, in which there are many cell types such as excitatory and inhibitory neurons. The cell-type specific labeling systems we have developed in this study contribute to elucidating molecular etiology of psychiatric disorders at a single cell level.
Amount: ¥6,500,000
Funding type: research
Funder scheme: Grant-in-Aid for Challenging Research (Exploratory)
Start date: 2017-06-30
End date: 2020-03-31
Investigator: Hitoshi Hashimoto
Institution: Osaka University
Funded outputs count: 16
Funder award ID: 17K19488
OpenAlex ID: G303839182