Contribution of protein recycling system for the maintenance of skeletal muscle mass
Description: Proteolysis has long been considered deleterious to skeletal muscle. Recent studies, however, have identified that impaired proteolytic function leads to loss instead of an increase in skeletal muscle mass. Dysfunction in the proteasome-dependent proteolytic pathway in skeletal muscle induced more significant loss or impaired development than dysfunction in the autophagy pathway. Although we failed to detect the recycling of degradation derived amino acids for de-novo protein synthesis, we found the induction of various aminopeptidases linked to the proteasome-dependent proteolytic pathway. Each aminopeptidase in cultured myoblasts demonstrated essential roles, such as the regulations of ATP concentration, cell cycle and initiation of differentiation, and determination of polarity and structure of myotubes. Although further studies are required to elucidate the underlying mechanisms, we could demonstrate the essential roles of post-proteolytic aminopeptidases in developing myoblasts.
Amount: ¥44,590,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (A)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Ryoichi Nagatomi
Institution: Tohoku University
Funded outputs count: 5
Funder award ID: 18H04080
OpenAlex ID: G3131467229