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Real-time observation of native conformations and molecular behaviors of viral fusion proteins using high-speed atomic force microscopy (HS-AFM)

Description: HS-AFM is an ideal tool for investigating properties of viral fusion proteins that include native conformation, binding activity with host factors, and structural dynamic during fusogenic transition. Furthermore, interaction between fusion proteins and exosomes will be also be recorded.
Amount: ¥4,160,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Early-Career Scientists
Start date: 2020-04-01
End date: 2024-03-31
Investigator: Kee Siang Lim
Institution: Kanazawa University
Funded outputs count: 11
Funder award ID: 20K16262
OpenAlex ID: G3310947669
Crafty mimicry grants nuclear pore entry to HIV
2024 · Keesiang Lim, Masaharu Hazawa, et al. · Cell Host & Microbe · 5
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Nanoscopic Elucidation of Spontaneous Self-Assembly of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Open Reading Frame 6 (ORF6) Protein
2023 · Goro Nishide, Keesiang Lim, et al. · The Journal of Physical Chemistry Letters · 15
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Recent advances in sensing the inter-biomolecular interactions at the nanoscale – A comprehensive review of AFM-based force spectroscopy
2023 · Anabel Lostao, KeeSiang Lim, et al. · International Journal of Biological Macromolecules · 93
Nanoscopic Assessment of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM
2023 · Keesiang Lim, Goro Nishide, et al. · Nano Letters · 19
Spatiotemporal tracking of small extracellular vesicle nanotopology in response to physicochemical stresses revealed by HS‐AFM
2022 · Elma Sakinatus Sajidah, Keesiang Lim, et al. · Journal of Extracellular Vesicles · 27
Discovery of a Novel Aminocyclopropenone Compound That Inhibits BRD4-Driven Nucleoporin NUP210 Expression and Attenuates Colorectal Cancer Growth
2022 · Hiroya Kondo, Kenji Mishiro, et al. · Cells · 20
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Millisecond dynamic of SARS‐CoV‐2 spike and its interaction with ACE2 receptor and small extracellular vesicles
2021 · Keesiang Lim, Goro Nishide, et al. · Journal of Extracellular Vesicles · 60
NSP9 of SARS-CoV-2 attenuates nuclear transport by hampering nucleoporin 62 dynamics and functions in host cells
2021 · Kei Makiyama, Masaharu Hazawa, et al. · Biochemical and Biophysical Research Communications · 34
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How SARS-CoV-2 and Other Viruses Build an Invasion Route to Hijack the Host Nucleocytoplasmic Trafficking System
2021 · Elma Sakinatus Sajidah, Keesiang Lim, et al. · Cells · 32
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High-Speed Atomic Force Microscopy Reveals Spatiotemporal Dynamics of Histone Protein H2A Involution by DNA Inchworming
2021 · Goro Nishide, Keesiang Lim, et al. · The Journal of Physical Chemistry Letters · 30
High-Speed AFM Reveals Molecular Dynamics of Human Influenza A Hemagglutinin and Its Interaction with Exosomes
2020 · Keesiang Lim, Noriyuki Kodera, et al. · Nano Letters · 37
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