Paracrine interactions among human cultured corneal endothlial cells regulated the ce state transition in a paracrine fashion
Description: MiRs in culture supernatants of HCECS were different from those selected in cHCECs. miR146, 34a, and 378 were the representatives of the latter. CD44 is the key to distinguishing differentiated cHCECs from either un-differentiated or cHCECs with CST. The miR378 showed the gradual decrease in parallel with decreases of CD44 expression. Of note, CD44 might be repressed by wild-type p53.It is intriguing that the selected secreted miRs by comparison of mature differentiated cHCECs versus cHCECS with senescent-like CST or versus cHCECs with distinct CD44 expression levels were mutually not overlapped,thus implicat- ing the distinct role in the maintenance of culture homeostasis. The observed elevated secretion of exosomes in CST-cHCECs might be under the regulation of p53. In regard to the secreted miRs, one of the most relevant issues that remains elusive is whether these secreted miRs are released from, and act on cHCECs as inclusion molecules in exosomes.
Amount: ¥16,120,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (B)
Start date: 2014-04-01
End date: 2017-03-31
Investigator: Shigeru Kinoshita
Institution: Kyoto Prefectural University of Medicine
Funded outputs count: 6
Funder award ID: 26293376
OpenAlex ID: G342985174