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Quantitative real-time characterization of single-cell aging: from phenotypes to

Description: PUBLIC HEALTH RELEVANCE: Using quantitative systems biology approaches and microfluidics platforms, I propose to investigate how single yeast cells replicatively age in real-time. Since a number of genetic and cell biological processes are conserved between yeast and higher eukaryotic cells, our results will contribute significant insights into understanding ho complex eukaryotic cells age, including human cells.

Funder: National Institute on Aging

Funding type: grant

Funder scheme: DP2

Start date: 2014-09-30

End date: 2019-05-31

Investigator: MURAT ACAR

Institution: YALE UNIVERSITY

Funded outputs count: 19

Funder award ID: DP2AG050461

OpenAlex ID: G2180622536

A yeast-optimized single-cell transcriptomics platform elucidates how mycophenolic acid and guanine alter global mRNA levels

2021 · Guste Urbonaite, Jimmy Tsz Hang Lee, et al. · Communications Biology · 22

Epigenetic Mechanisms Contribute to Evolutionary Adaptation of Gene Network Activity under Environmental Selection

2020 · Xinyue Luo, Ruijie Song, et al. · Cell Reports · 17

Characterization of the impact of GMP/GDP synthesis inhibition on replicative lifespan extension in yeast

2020 · Ping Liu, Ethan A. Sarnoski, et al. · Current Genetics · 4

Quantitative Insights into Age-Associated DNA-Repair Inefficiency in Single Cells

2019 · Thomas Z. Young, Ping Liu, et al. · Cell Reports · 17

Stochastic modeling of aging cells reveals how damage accumulation, repair, and cell-division asymmetry affect clonal senescence and population fitness

2019 · Ruijie Song, Murat Acar · BMC Bioinformatics · 21

Inhibition of GMP synthesis extends yeast replicative lifespan

2019 · E. A. Sarnoski, P. Liu, et al. · bioRxiv (Cold Spring Harbor Laboratory)

Insights into Bidirectional Gene Expression Control Using the Canonical GAL1/GAL10 Promoter

2018 · Gregory L. Elison, Yuan Xue, et al. · Cell Reports · 27

Multi-component gene network design as a survival strategy in diverse environments

2018 · Xinyue Luo, Ruijie Song, et al. · BMC Systems Biology · 6

The Systems Biology of Single-Cell Aging

2018 · Ruijie Song, Ethan A. Sarnoski, et al. · iScience · 30

Fundamental Characteristics of Single-Cell Aging in Diploid Yeast

2018 · Ethan A. Sarnoski, Ruijie Song, et al. · iScience · 36

Scarless genome editing: progress towards understanding genotype–phenotype relationships

2018 · Gregory L. Elison, Murat Acar · Current Genetics · 8

Live-Cell Imaging of Chromatin Condensation Dynamics by CRISPR

2018 · Yuan Xue, Murat Acar · iScience · 35

Mechanisms for the epigenetic inheritance of stress response in single cells

2018 · Yuan Xue, Murat Acar · Current Genetics · 34

Heritable stress response dynamics revealed by single-cell genealogy

2018 · Meenakshi Chatterjee, Murat Acar · Science Advances · 21

The generational scalability of single-cell replicative aging

2018 · Ping Liu, Murat Acar · Science Advances · 27

A High-Throughput Screen for Yeast Replicative Lifespan Identifies Lifespan-Extending Compounds

2017 · Ethan A. Sarnoski, Ping Liu, et al. · Cell Reports · 34

Noise reduction as an emergent property of single-cell aging

2017 · Ping Liu, Ruijie Song, et al. · Nature Communications · 37

A Precise Genome Editing Method Reveals Insights into the Activity of Eukaryotic Promoters

2017 · Gregory L. Elison, Ruijie Song, et al. · Cell Reports · 9

Yeast Replicator: A High-Throughput Multiplexed Microfluidics Platform for Automated Measurements of Single-Cell Aging

2015 · Ping Liu, Thomas Z. Young, et al. · Cell Reports · 79