Cytoplasmic acetylation regulates signal transduction and B cell development
Description: Interleukin-7 (IL-7) is essential for lymphocyte development. To identify functional subdomains in the cytoplasmic tail of IL-7 receptor alpha (IL-7Ra) chain, here, we constructed a series of IL-7Ra deletion mutants. We found that IL-7Ra-deficient hematopoietic progenitor cells (HSCs) gave rise to B cells both in vitro and in vivo when a wild-type (WT) IL-7Ra chain was introduced; however, no B cells were observed under the same conditions from IL-7Ra-deficient HSCs with introduction of exogenous IL-7Ra subunit, which lacked the amino acid region at position 414-441 (d414-441 mutant). Signal transducer and activator of transcription 5 (Stat5) was phosphorylated in cells with d414-441 mutant, similar to that in WT cells, in response to IL-7 stimulation. In contrast, more truncated Stat5 was generated in cells with d414-441 mutant in WT cells. These results suggested that amino acid 414-441 in IL-7Rα chain formed a critical subdomain necessary for role of IL-7 in B cell development.
Amount: ¥4,810,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2017-04-01
End date: 2021-03-31
Investigator: Taku KUWABARA
Institution: Toho University
Funded outputs count: 7
Funder award ID: 17K08892
OpenAlex ID: G3781067503