Establishment of differentiation induction method from ES cells to inner ear progenitor cells by Tlx3 and application to inner ear regenerative medicine
Description: TLX3 is expressed in cells whose fate is determined to be inner ear progenitor cells and is considered to be an excellent marker for inducing differentiation into inner ear progenitor cells.To establish the methods for selecting inner ear progenitor cells, we produced mouse ES cells in which a reporter gene (= eGFP) was knocked in at the TLX3 locus. In these ES cells, however, GFP expression was not observed even after incubation under differentiation conditions.Neurogenin2 is also considered to be an excellent marker for inducing differentiation into inner ear progenitor cells. We, therefore, produced mouse ES cells in which a reporter gene (= eGFP) was knocked in at the Neurogenin2 locus. and in these ES cells, eGFP expression was observed after incubation under differentiation conditions.Using this mouse ES cells, we are now trying to establish the methods for selecting inner ear progenitor cells.
Amount: ¥4,680,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2017-04-01
End date: 2020-03-31
Investigator: Hata Ryuji
Institution: Fujita Health University
Funded outputs count: 0
Funder award ID: 17K11344
OpenAlex ID: G3848463178