Mechanism of transcription coupled to RNA processing and its defect
Description: This research aimed to understand a gene transcription and its coupled RNA processing, which are important regulatory steps in eukaryotic gene expression, and to develop necessary analytical methods and gain medical insights. Firstly, we successfully established the POINT method, which allows analysis of newly transcribed RNA. My POINT method reveals precise transcription start sites, RNA cleavage sites, and kinetics of co-transcriptional splicing. We also demonstrates the importance of alternative splicing during developmental stages. Additionally, the mechanisms of transcription termination defect in colorectal cancer and kidney cancer are revealed. The findings of this research contribute to the advancement of basic biology and also the development of treatment methods for diseases such as cancer.
Amount: ¥55,770,000
Funding type: research
Funder scheme: Fund for the Promotion of Joint International Research (Home-Returning Researcher Development Research)
Start date: 2021-03-12
End date: 2024-03-31
Investigator: Takayuki Nojima
Institution: Kyushu University
Funded outputs count: 8
Funder award ID: 19K24692
OpenAlex ID: G3949150889