Novel three-dimensional bone regenerative medicine strategy using human dental pulp stem cells
Description: Establishment of a highly efficient bone differentiation induction method by using helioxanthin derivative (TH), which is a small chemical compound having osteoblast-inducing ability for human dental pulp stem cells (DPSCs), and further using a DPSC sheet prepared with TH-added medium The bone regeneration ability in vivo was examined.The ability of DPSCs to induce bone differentiation by TH in vitro was examined, and it was found that the expression of bone differentiation markers was most significantly increased in the TH (10-6 M) group, and that bone differentiation was possible by short-term culture with TH. Furthermore, DPSCs sheets were prepared in TH-added bone differentiation induction medium and transplanted to calvarial bone defect of mice, and extensive bone regeneration was observed in the in vivo group.
Amount: ¥4,810,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: Kawase-Koga YOKO
Institution: Tokyo Medical University
Funded outputs count: 4
Funder award ID: 16K11700
OpenAlex ID: G404001926