Evolutionary loss of regenerative ability: The role of evolutionary conserved regeneration signal-response enhancer
Description: We screened regeneration signal-response enhancers (RSREs) at the lhx1 locus using a Xenopus transgenic system and found that the noncoding elements conserved between only highly regenerative species do not show strong enhancer activities in the regenerating amphibian nephric duct. Instead, the noncoding elements conserved from fish to humans function as enhancers in the regenerating nephric duct. We also found that DNA-binding motif of Arid3a, a component of H3K9me3 demethylases, was commonly found in RSREs. Arid3a is known as a component of H3K9 me3 demethylases KDM4/JMJD2 complex. Arid3a binds to RSREs and reduces the H3K9me3 levels on RSREs. It also promotes cell cycle progression and causes the outgrowth of nephric tubules, whereas the conditional knockdown of arid3a using photo-morpholino inhibits regeneration. These results suggest that Arid3a contributes to the regeneration of nephric tubules by decreasing H3K9me3 on RSREs.
Amount: ¥4,940,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: Ochi Haruki
Institution: Yamagata University
Funded outputs count: 8
Funder award ID: 16K07362
OpenAlex ID: G4131104746