Induction of human iTS-P and iTS-L cells using iPS cell technology
Description: We recently demonstrated the generation of mouse induced tissue-specific stem (iTS) cells through transient overexpression of reprogramming factors combined with tissue-specific selection. Here we induced expandable tissue-specific stem/progenitor (iTS) cells from human pancreatic/hepatic tissue through transient expression of genes encoding the reprogramming factors OCT4, p53 shRNA, SOX2, KLF4, L-MYC, and LIN28.Transfection of episomal plasmid vectors into human pancreatic tissue efficiently generated iTS cells expressing genetic markers of endoderm and tissue-specific progenitors. The iTS cells differentiated into insulin/albumin-producing cells more efficiently than human iPS cells. iTS cells subcutaneously inoculated into immunodeficient mice did not form teratomas. The generation of human iTS cells may have important implications for the clinical application of stem/progenitor cells.
Amount: ¥17,420,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (B)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: Hirofumi NOGUCHI
Institution: University of the Ryukyus
Funded outputs count: 23
Funder award ID: 16H05404
OpenAlex ID: G4236009681