Elucidate molecular communication between hematopoietc stem cells and their niche by definitive gene expression profiling
Description: This study aims to elucidate the molecular communication between hematopoietic stem cells and the cells that forming the surrounding microenvironment using comprehensive gene expression profiling. Since the standard method for gene expression profiling itself evloved drastically immediately after the application, we first aimed to establish single-cell RNA-seq technology.As a result, we succeeded in developing a sample processing pipeline and a data analysis pipeline based on the SMARTseq method that can perform the world's highest level of single-cell RNA-seq. Currently, we have succeeded in quantitatively and stably detecting the expression of approximately 11,000 genes from a single cell.
Amount: ¥4,550,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Jun Seita
Institution: Institute of Physical and Chemical Research
Funded outputs count: 2
Funder award ID: 18K08377
OpenAlex ID: G4430098774