Myosin dynamics in cultured skeletal muscle cells and isolated fibers
Description: In skeletal muscles, myosin is a major component of myofibrillar proteins. In myofibrils, approximately 300 myosin molecules form a single thick filament where continuous turnover of myosin is observed. However, we have not fully understood the mechanism by which myosin replacement is regulated in skeletal muscle cells. Here, we showed that one of the sources for myosin substitution in myofibrils was stored myosin in the cytoplasm of myotubes. Next, we searched for factors to affect myosin replacement rate in myofibrils. Our results demonstrated that chaperone activity of Hsp90 increased myosin replacement rate through upregulation of myosin amount in cultured myotubes. Finally, we generated genetically modified mouse in which red fluorescence protein fused myosin heavy chain was expressed in skeletal muscle cells to monitor the dynamics of endogenous myosin.
Amount: ¥4,810,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2016-04-01
End date: 2020-03-31
Investigator: Koichi OJIMA
Institution: National Agriculture and Food Research Organization
Funded outputs count: 4
Funder award ID: 16K08079
OpenAlex ID: G4534968965