Regeneration of dentin-pulp complex from iPS cells using new odontoblasts differentiation mechanism and polarity regulation
Description: The aim of this study was to develop a technology to induce dentin-pulp complex formation from human iPS cells (hiPS) by using human Copine-7 (hCPNE7) and Semaphorin4D, which regulates cell polarity. MSCs) induced from hiPS and miPS cells were differentiated into odontoblasts when exposed to hCPNE7 or hCPNE7-expressing HAT7 (rat enamel blast cell line) culture supernatant. Furthermore, the basement membrane component secreted by the newly established Hertwig epithelial sheath cell line (HERS02T) also showed high induction of odontblast differentiation and polarity toward mouse incisor papillary mesenchymal stem cells, and similar results were obtained for NCLCs. The combined 3D culture of epithelial cell organoids and NCLC organoids showed enhanced differentiation induction and calcification formation.
Amount: ¥16,510,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (B)
Start date: 2018-04-01
End date: 2022-03-31
Investigator: Hidemitsu Harada
Institution: Iwate Medical University
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Funded outputs count: 18
Funder award ID: 18H02984
OpenAlex ID: G4584589865