Effect of truncated Hepatitis B virus X protein integration on malignant potential of HCC
Description: Recently GWAS about HBV-related HCC revealed that COOH-truncated HBx was integrated in host genome at high frequency. We aimed to analyze the relationship between COOH-truncated HBx expression and CSCs which is related to malignant potential. COOH-truncated HBx(truncated HBx/HepG2) shows significantly resistant to anti-tumor agent, 5-FU. Soft gel agar colony formation assay shows that truncated-HBx/HepG2 cells make significantly more colonies than control. Flow cytometry analysis reveals that truncated HBx/HepG2 cells express high levels of CD44, one of cancer stemness maker. Subsequently we investigated what factor up-regulate CD44 in truncated HBx/HepG2 cells by Human Stem Cell PCR Array (Qiagen).This PCR-array results indicated that LTBP1 is significantly up-regulated in only Truncated-HBx/HepG2 . The expression level of CD44 were significantly down-regulated by siRNA knockdown of LTBP1. In sorted CD44high cells, LTBP1 were significantly up-regulated compared with CD44low cells.
Amount: ¥4,680,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: Suda Goki
Institution: Hokkaido University
Funded outputs count: 17
Funder award ID: 16K09334
OpenAlex ID: G4703580585