Somatic cell nuclear reprogramming by maternal factors
Description: Mammalian mature oocytes have the ability to reprogram the epigenomic information of the terminally differentiated cell to reacquire totipotency. In this study, we aimed to develop a method for generating high-quality iPS cells using a set of genes specifically expressed in totipotent cells (totipotent cell-specific genes). We revealed that some genes increase and some decrease the efficiency of iPS cell derivation. We found that some genes increased the percentage of Nanog-positive iPS cells compared to that of control iPS cells when they were expressed only in the early stages of iPS cell induction. In the future, we plan to investigate the quality of the generated iPS cells, including their differentiation ability.
Amount: ¥6,240,000
Funding type: research
Funder scheme: Grant-in-Aid for Challenging Research (Exploratory)
Start date: 2018-06-29
End date: 2020-03-31
Investigator: Toshinobu Nakamura
Institution: Nagahama Institute of Bio-Science and Technology
Funded outputs count: 1
Funder award ID: 18K19188
OpenAlex ID: G5124468488