Identification of novel methylation markers in HPV-associated oropharyngeal cancer for precision medicine
Description: Genome-wide discovery using RNA sequencing and reduced representation bisulfite sequencing yielded 21 candidates for methylation-targeted genes. A verification study using Q-MSP identified 10 genes that showed an increase recurrence in methylation groups with oropharyngeal cancer (OPC). Further study on ctDNA in HPV-associated OPC showed that three genes (CALML5, DNAJC5G, and LY6D) had a high predictive ability as emerging biomarkers for a validation set, each capable of discriminating between the plasma of the patients from healthy individuals. Among pre-treatment ctDNA samples, methylated CALML5, DNAJC5G, and LY6D were observed in 8/8 (100%), 7/8 (87.5%), and 7/8 (87.5%) samples, respectively. Methylated CALML5, DNAJC5G, and LY6D were found in 2/8 (25.0%), 0/8 (0%), and 1/8 (12.5%) of the final samples in the series, respectively. Here, we present the relationship between the methylation status of three genes and cancer recurrence for risk classification of HPV-associated OPC cases.
Amount: ¥4,420,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (B)
Start date: 2017-04-01
End date: 2020-03-31
Investigator: Atsushi Imai
Institution: Hamamatsu University School of Medicine
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 14
Funder award ID: 17K16904
OpenAlex ID: G5453785210