Production of novel prebiotics for intestinal bifidobacteria from food wastes
Description: The production of prebiotics for intestinal bifidobacteria from two food wastes was studied.(1) By using bifidobacterial glycosidases or cells, bifidogenic disaccharide galacto-N-biose was released from wasted mucin, which contains abundant O-glycans. A novel 6-sulfo-N-acetylglucosaminidase, an essential enzyme for degradation of terminally sulfated glycans, was identified. A bifidobacterial strain defective in incorporation of galacto-N-biose was also screened, which is suitable for production of the disaccharide.(2) Mume-polyphenols prepared from umesu, a waste of umeboshi production, was found to enhance bifidobacterial growth. The major phenolic compound, prunose, was assimilated by B. catenulatum and B. pseudocatenulatum, which express prunose-specific ferulate esterase. Arabinan-like polysaccharides was degraded by two novel GH43 exo-α-arabinofuranosidases from B. longum. Effects of orally administrated mume-polyphenols on intestinal microbiota was evaluated using mice.
Amount: ¥4,940,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2015-04-01
End date: 2018-03-31
Investigator: Hisashi Ashida
Institution: Kindai University
Funded outputs count: 13
Funder award ID: 15K07448
OpenAlex ID: G5565394105