Development of iPS cell-based tooth regeneration techniques by a transposon-based gene delivery system
Description: The aim of this study was to develop induced pluripotent stem (iPS) cell-based tooth regeneration techniques by a transposon-based tetracycline (tet)-controlled gene regulation system. We focused on BMP-4, an important gene for tooth germ development, and successfully established a tet-controlled BMP-4 gene regulation system for iPS cells in which transcriptional activation of BMP-4 was associated with enhanced mesodermal and non-neuroectodermal lineage commitment. These findings represent an important step toward genetically engineered self-organization of iPS cells, particularly for generation of mesodermal cartilage and non-neuroectodermal organs, such as teeth.
Amount: ¥17,160,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (B)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: Hiroshi Egusa
Institution: Tohoku University
Funded outputs count: 6
Funder award ID: 16H05519
OpenAlex ID: G5871512128