Development of osteoblast culture system for bone regenerative treatment for cell transplantation
Description: In recent years, three-dimensional culture with a hydrogel that is flexible and easily mimics a living environment for cell transplantation treatment has been actively studied in recent years. The research representative examined the influence of the culture environment on the cells by performing three-dimensional culture using Gelatin Metacryloyl (GelMA), which is a highly compatible and highly cell-adhesive biomaterial. Since GelMA changes hardness by concentration, we analyzed hardness optimized for osteoblasts, and as a result, 16 Kpa was optimum. As a result of culturing at this hardness, the cells aggregated and an increase in expression of bone matrix protein gene was observed. From these facts, it was found that three-dimensional culture by GelMA is suitable for culture toward bone tissue regeneration.
Amount: ¥2,600,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (B)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: Makoto Aino
Institution: Aichi Gakuin University
Funded outputs count: 3
Funder award ID: 16K20684
OpenAlex ID: G5961834785