Identification of reprogramming factors using the nuclear transfer system specialized for analysing transcriptional reprogramming
Description: The somatic cell nucleus is reprogrammed by transplanting it into an egg, and embryonic/pluripotency genes are activated in the nuclear transfer embryos, finally culminating in the creation of embryonic cells. However, an effective experimental system for identifying embryonic factors directly involved in this transcriptional reprogramming has not been established, and little is known about it. In this research, we have developed a nuclear transfer system specialized for the analysis of transcription reprogramming. Using this nuclear transfer method, we succeeded in directly reprogramming a somatic cell nucleus to a mouse early embryo-like state in terms of the epigenetic modification and the transcriptional state. Furthermore, we detected embryonic gene activation from interspecies donor cells in mouse embryos, which has been believed to be difficult with a conventional nuclear transfer method.
Amount: ¥24,830,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (A)
Start date: 2017-04-01
End date: 2021-03-31
Investigator: Kei Miyamoto
Institution: Kindai University
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 22
Funder award ID: 17H05045
OpenAlex ID: G6213207692