Periodontal resgeneration using co-cultured spheroids of hPDLMSCs and HUVECs
Description: We investigated the characteristics and potential of periodontal tissue regeneration in co-cultured spheroids of hPDLMSCs and HUVECs in vitro and in vivo. The expression levels of stemness markers, vascular endothelial growth factor (VEGF), and osteogenesis-related genes were up-regulated in co-cultured spheroids, compared with monolayer and spheroid cultures of hPDLMSCs. The nodule formation was increased in co-cultured spheroids, compared with monolayer and spheroid-cultured hPDLMSCs. Periodontal tissue defects were prepared in the maxillary first molars of rats and subjected to transplantation assay. Treatment with co-cultured spheroids enhanced new cementum formation after 4 or 8 weeks of transplantation, although there was no significant difference in the new bone formation between co-cultured spheroids and hPDLMSC spheroids. These data suggest that co-cultured spheroids enhance the periodontal tissue regeneration.
Amount: ¥4,680,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: MICHIHIKO USUI
Institution: Kyushu Dental College
Funded outputs count: 4
Funder award ID: 16K11838
OpenAlex ID: G6311770581