Transplantation of small intestinal organoids onto the colon and its potential as a novel therapeutic approach for intestinal failure
Description: Extensive resection of the small intestine (SI) leads to severe short bowel syndrome (SBS). To explore a new approach for regenerative medicine for this condition, we have developed in this study a mouse model of local replacement of the colon epithelium with the SI one, by removing the colonic mucosa with chelating agents and subsequently transplanting SI organoids. We found that adult SI organoids regenerate new epithelia that morphologically preserve mature SI phenotypes even in the colonic environment after engraftment. Molecular as well as histological analyses confirmed the preservation of gene and protein expression patterns of SI epithelial cells in the ectopically grafted tissue. The results of our study suggest that the mesenchymal component of the remnant colon in SBS patients would provide a scaffold for the SI organoid transplantation, which may compensate for the loss of function of the SI in SBS patients.
Amount: ¥17,420,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (B)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Nakamura Tetsuya
Institution: Juntendo University(2019-2020)Tokyo Medical and Dental University(2018)
Funded outputs count: 5
Funder award ID: 18H02867
OpenAlex ID: G6358453236