Analysis of cleavage fragments produced by proteolysis on cancer cell membrane
Description: An early detection method for pancreatic cancer has not been established so far. In this study, we focused on the proteolytic fragment of substrates of membrane-type-1 matrix metalloprotease (MT1-MMP), which is important for the malignant progression of pancreatic. In this study, we made monoclonal antibodies to the proteolytic fragments of MT1-MMP substrates, heparin-binding EGF-like growth factor, HB-EGF and EphA2, and established quantitative ELISA assays for the fragments. As the results, the detection sensitivity of the HB-EGF fragment was not sufficient and required further improvement. In contrast, the serum levels of EphA2 fragment in the patient with pancreatic cancer including an early stage was a significantly higher than that of healthy donors. These results suggest that the EphA2 fragment could be a new biomarker for early pancreatic cancer diagnosis.
Amount: ¥4,680,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2017-04-01
End date: 2020-03-31
Investigator: NAOHIKO KOSHIKAWA
Institution: Kanagawa Cancer Center Research Institute
Funded outputs count: 12
Funder award ID: 17K09027
OpenAlex ID: G6922533697