Genome-wide analysis of mRNA-selective translation by initiation factor paralogs
Description: Eukaryotic translation initiation factor (eIF) 4A, a DEAD-box RNA-binding protein, plays an essential role in translation initiation. Two mammalian eIF4A paralogs, eIF4A1 and eIF4A2, have been assumed to be redundant because of their high homology, and the difference in their functions has been poorly understood. Here, combining multiple genome-wide methods, we found that eIF4A paralogs have preferential mRNA binders and that the bias originates from RNA binding protein LARP1, which binds to eIF4A1 selectively. Moreover, we revealed that eIF4A1 enhances the affinity between TOP mRNAs and LARP1 and thus ensures stronger translation repression of TOP mRNAs upon mTORC1 inhibition.
Amount: ¥26,130,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (A)
Start date: 2017-04-01
End date: 2021-03-31
Investigator: Shintaro Iwasaki
Institution: Institute of Physical and Chemical Research
Funded outputs count: 41
Funder award ID: 17H04998
OpenAlex ID: G7043491024