Establishment of AD model mice harboring novel pathogenic PS1 mutation presenting "Behavioral and Psychological Symptoms of Dementia"
Description: We report the familial Alzheimer pedigree carrying a novel mutation, three bases deletion, which results in one amino acid deletion of PS1 (Psen1 delta p.277). To establish the adequate animal model of AD especially presenting BPSD-like symptoms, we planned to make the transgenic mice harboring the mutation using CRISPR/Cas9 method. Even after injecting more than 200 times, no living mutant mouse was obtained. Remodeling guide RNA(crRNA) results in successfully establishing the transgenic mice, which will enable the neuropathological and behavioral analysis after aging of these transgenic mice, both heterozygote and possibly homozygote.
Amount: ¥4,810,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2015-04-01
End date: 2018-03-31
Investigator: Jun Kawamata
Institution: Sapporo Medical University
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Funded outputs count: 7
Funder award ID: 15K09840
OpenAlex ID: G7062848281