Influence of circadian rhythm on the susceptibility of the inner ear
Description: Immunostaining of the brain tissue and cochlea was performed at the circadian time when the expression level of circadian rhythm protein was the highest. Brain tissues were subjected to free floating sections, frozen sections, and paraffin sections, and the cochlea to surface preparation, frozen sections and paraffin sections. Only PER1 and PER2 positive cells could be observed in the suprachiasmatic nucleus of the floating section of the brain. The expression of the circadian protein in the cochlea was difficult to evaluate because of the autofluorescence. In another experiment, C57BL / 6J was exposed to intense sound. The ABR thresholds were greater in animals exposed to noise in the daytime than those exposed in the nighttime both 24 hours and 2 weeks after noise exposure, which was inconsistent with the findings in a previous study.
Amount: ¥6,240,000
Funding type: research
Funder scheme: Grant-in-Aid for Challenging Research (Exploratory)
Start date: 2018-06-29
End date: 2020-03-31
Investigator: Tatsuya Yamasoba (lead)
Institution: The University of Tokyo
Funded outputs count: 4
Funder award ID: 18K19602
OpenAlex ID: G7156569446