Search for protein factors involved in mammalian mtDNA replication
Description: Most of a cell’s energy is generated in the compartment of the cell called mitochondria. Mitochondria contain a small circle of DNA, called mitochondrial DNA (mtDNA), that is essential for energy production to maintain life. The significance of mtDNA maintenance can be recognized by the fact that abnormalities in mtDNA are associated with human pathology. A deeper understanding of how mtDNA is replicated and maintained is thus important for the progress of bioscience and medicine. This research project was aimed at finding a new protein factor(s) that are involved in the replication and/or maintenance of mtDNA. To this end, methods for purifying mtDNA-protein complexes were constructed. Then attempts were made to capture proteins that were contained in the complexes, followed by determination of the identity of the proteins. Further, whether the proteins are involved in mtDNA replication or maintenance were examined.
Amount: ¥4,160,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (B)
Start date: 2014-04-01
End date: 2017-03-31
Investigator: Takehiro Yasukawa
Institution: Kyushu University
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 3
Funder award ID: 26840011
OpenAlex ID: G7230973450