Regulation mechaninism of DNA damage response and DNA focus formation during neural tissue development.
Description: Using transgenic mice expressing EGFP-53BP1 fusion fluorescent protein that accumulates at DNA damage sites, we created and analyzed mice to detect DNA damage focus formation, one of a DNA damage response (DDR) in hippocampal neurons. In hippocampal neurons within the tissue, focus formation was suppressed (Off), but when these cells were dissociated and cultured as primary cultures, focus formation resumed (On). This suggests that in highly differentiated neurons within tissue, DDR is suppressed, whereas in primary cultures where the cells revert to a less differentiated state, DDR is reactivated.
Amount: ¥4,290,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2018-04-01
End date: 2024-03-31
Investigator: Akihiro Kurimasa (lead)
Institution: Tohoku Medical and Pharmaceutical University
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 9
Funder award ID: 18K07727
OpenAlex ID: G7387139654