Validation and exploration of stem cell differentiation and immune characteristics for use in regenerative cellular therapeutics
Description: Observation of cardiomyocyte differentiation from stem cells revealed that the cells became confluent about 4 days after differentiation induction treatment, and that small autonomously beating nodes were evident by 10 days. The timing of the appearance of autonomously beating nodes and their number differed among cell lines and sublines. Secretion of vascular endothelial growth factor by human mesenchymal stem cells under ischemic conditions varied among the stem cell line lot numbers. As this difference in secretory capacity may affect the degree of angiogenesis and therapeutic efficacy after stem cell transplantation, and we explored factors that might predict the repair capacity of cells. In addition, analysis of the immunological behavior of mesenchymal stem cells revealed no immune effect on them. As it will be important to develop a highly accurate assay method for detection of abnormal cells, the safety of cell and tissue processing products was also investigated.
Amount: ¥4,940,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2016-04-01
End date: 2019-03-31
Investigator: Mitsutoshi Satoh
Institution: Meiji Pharmaceutical University
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Funded outputs count: 0
Funder award ID: 16K08382
OpenAlex ID: G739921842