Development of novel Mg2+ imaging technique and measurement of Mg2+ concentration changes in cultured glial cells.
Description: In this study, I developed novel technique to measure intracellular Mg2+ concentration changes based on fluorescent resonance energy transfer (FRET) between a fluorescent protein and KMG-104-AsH, which is a Mg2+-selective fluorescent probe we developed before. This technique enables us to quantitatively visualize changes in Mg2+ concentration in intracellular specific areas on demand.By using it and other KMG probes, I checked whether neurotransmitters affect Mg2+ concentration in astrocyte, which is a kind of glial cells. I demonstrated that GABA and serotonin induce changes in Mg2+ concentration in cultured rat astrocytes.
Amount: ¥3,900,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Young Scientists (B)
Start date: 2017-04-01
End date: 2019-03-31
Investigator: Yutaka Shindo
Institution: Keio University
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Funded outputs count: 3
Funder award ID: 17K13268
OpenAlex ID: G7419409142