The development of novel desensitization therapy for transplant patients with donor specific HLA antibody.
Description: In this study, we investigated the effect of anti-CD20 mAb -mediated B-cell depletion on anti-donor T-cell responses and the inhibitory role of B-cells in a highly sensitized transplant model. The average stimulation index values for CD4+ T-cell responses to donor stimulation were significantly higher in the desensitization group than those in the control group. When co-cultured with various B-cells in the CFSE-MLR assay of the desensitization group, the addition of whole CD19+ B-cells and B1-cells significantly suppressed the anti-donor CD4+ T-cell responses. However, the addition of non B1-cells did not suppress the anti-donor CD4+ T-cell responses. These findings demonstrate that B-cell depletion with anti-CD20 mAb exacerbates anti-donor CD4+ T-cell responses in a highly sensitized transplant model. It might be possible that the sensitized CD5+ CD19+ B1-cells have an ability to inhibit anti-donor CD4+ T-cell responses.
Amount: ¥4,680,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2017-04-01
End date: 2020-03-31
Investigator: Kentaro Ide
Institution: Hiroshima University
Funded outputs count: 4
Funder award ID: 17K10512
OpenAlex ID: G7515045083