Analysis of neural and molecular mechanism of sleep fragmentation with aging in Drosophila
Description: We obtained a typical sigmoidal survival curve with half-lethal of 12 weeks and a maximum lifespan of 17 weeks in females while males are dead monotonically with aging up to 12 weeks. Then we compared 1-week and 10-week-old males focused on the locomotor activities recorded by 1 second bin. The sleep fragmentation was shown every 45 minutes in the old flies.We sampled 1 and 10-week-old male brains every 6 hours for 3 days, and performed RNAseq analyses. The bioinformatics analysis suggests that 8 to 10 clusters exist in cyclically expressing genes. Each cluster had a characteristic gene function and, in some cases, a common transcription factor. Based on these results, we identified candidate genes those likely to act as the primary cause of aging affecting cyclic gene expression, although a further analysis are necessary to identify how these genes functions in sleep fragmentation in Drosophila neural network.
Amount: ¥4,940,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2016-04-01
End date: 2020-03-31
Investigator: Akira Matsumoto
Institution: Juntendo University
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Funded outputs count: 0
Funder award ID: 16K07444
OpenAlex ID: G7516647809