Elucidation of the role of the cytoskeleton in mitochondrial dysfunction in cochlear cells
Description: The mitochondrial movement in the cochlea cell line (HEI-OC1) was continuously tracked by extracting feature points and calculating optical flow using OpenCV, calculating diffusion coefficients, etc. The same coefficients were calculated after exposure to cytoskeletal inhibitors, and dynamic evaluation was performed. The mitochondrial membrane potential was evaluated by plate reader and fluorescence microscopy. The morphology of mitochondria was evaluated by electron microscopy. After exposure to PQQ (0.1, 1.0 nM) for 1 day, the cells were exposed to hydrogen peroxide (100 μM) for 1 hour to evaluate mitochondrial function and mitochondrial area. SIRT1/PGC-1α pathway functions were evaluated.
Amount: ¥4,160,000
Funding type: fellowship
Funder scheme: Grant-in-Aid for Early-Career Scientists
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Teru Kamogashira (lead)
Institution: The University of Tokyo
Funded outputs count: 13
Funder award ID: 18K16906
OpenAlex ID: G755657400