Study for wound epidermis signal to regulate regeneration
Description: Wound epidermis (WE) and the apical epithelial cap (AEC) are believed to trigger regeneration of amputated appendages such as limb and tail in amphibians by producing certain secreted signaling molecules. Here we used an es1:egfp transgenic Xenopus laevis to isolate WE/AEC cells during the time course of tail regeneration. Transcriptome analysis revealed that more than 8,000 genes, including genes involved in several signaling pathways, displayed dynamic changes of their expression during tail regeneration. We identify seven secreted signaling molecule genes (mdk, fstl, slit1, tgfβ1, bmp7.1, angptl2 and egfl6) that are highly expressed in tail AEC cells. Among these genes, five were also highly expressed in limb AEC cells but the other two are specifically expressed in tail AEC cells. Interestingly, there was no expression of fgf8 in tail WE/AEC cells, whose expression and pivotal role in limb AEC cells have been reported previously.
Amount: ¥4,420,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2018-04-01
End date: 2022-03-31
Investigator: Makoto Mochii
Institution: University of Hyogo
Funded outputs count: 5
Funder award ID: 18K06266
OpenAlex ID: G7686104243