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Modeling human hepatotropic infections in complex tissue organoids

Description: Hepatitis B and C viruses are leading causes of human liver disease including cancer. This proposal aims to create and utilize novel primary human hepatocyte tissue culture systems and complex 3D liver organoids to study HCV and HBV mono- and co-infections. The resulting findings should aid development of more effective treatments aimed at eradicating these deadly viral diseases.

Funder: National Institute of Diabetes and Digestive and Kidney Diseases

Funding type: research

Funder scheme: R01

Start date: 2009-09-30

End date: 2015-08-31

Investigator: SANGEETA N. BHATIA

Institution: ROCKEFELLER UNIVERSITY

Topic: Liver physiology and pathology

Subfield: Hepatology

Field: Medicine

Domain: Health Sciences

Funded outputs count: 65

Funder award ID: R01DK085713

OpenAlex ID: G3701088827

Targeting BCL-XL in fibrolamellar hepatocellular carcinoma

2022 · Bassem Shebl, Denise Ng, et al. · JCI Insight · 28

Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice

2022 · Mohammad Kabbani, Eleftherios Michailidis, et al. · Cell Reports · 34

Mouse characteristics that affect establishing xenografts from hepatocellular carcinoma patient biopsies in the United States

2021 · Chenhui Zou, Imane El Dika, et al. · Cancer Medicine · 3

Identification of Novel Therapeutic Targets for Fibrolamellar Carcinoma Using Patient-Derived Xenografts and Direct-from-Patient Screening

2021 · Gadi Lalazar, David Requena, et al. · Cancer Discovery · 47

Human hepatocyte PNPLA3 148M exacerbates rapid non-alcoholic steatohepatitis development in chimeric mice

2020 · Mohammad Kabbani, Eleftherios Michailidis, et al. · bioRxiv (Cold Spring Harbor Laboratory) · 2

A Combination of Human Broadly Neutralizing Antibodies against Hepatitis B Virus HBsAg with Distinct Epitopes Suppresses Escape Mutations

2020 · Qiao Wang, Eleftherios Michailidis, et al. · Cell Host & Microbe · 75

Public broadly neutralizing antibodies against hepatitis B virus in individuals with elite serologic activity

2020 · Qiao Wang, Eleftherios Michailidis, et al. · bioRxiv (Cold Spring Harbor Laboratory) · 1

Expansion, in vivo–ex vivo cycling, and genetic manipulation of primary human hepatocytes

2020 · Eleftherios Michailidis, Koen Vercauteren, et al. · Proceedings of the National Academy of Sciences · 65

Co-Designing a Collaborative Chronic Care Network (C3N) for Inflammatory Bowel Disease: Development of Methods

2018 · Michael Seid, George Dellal, et al. · JMIR Human Factors · 38

Pan-Genotype Hepatitis E Virus Replication in Stem Cell–Derived Hepatocellular Systems

2017 · Xianfang Wu, Viet Loan Dao Thi, et al. · Gastroenterology · 71

Analysis of Hepatitis C Virus Particle Heterogeneity in Immunodeficient Human Liver Chimeric fah-/- Mice

2017 · Ursula Andreo, Ype P. de Jong, et al. · Cellular and Molecular Gastroenterology and Hepatology · 10

In situ expansion of engineered human liver tissue in a mouse model of chronic liver disease

2017 · Kelly R. Stevens, Margaret A. Scull, et al. · Science Translational Medicine · 164

Humanized mice efficiently engrafted with fetal hepatoblasts and syngeneic immune cells develop human monocytes and NK cells

2016 · Eva Billerbeck, Michiel C. Mommersteeg, et al. · Journal of Hepatology · 87

Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies

2016 · Vyas Ramanan, Kartik Trehan, et al. · Virology · 17

Superior In vivo Transduction of Human Hepatocytes Using Engineered AAV3 Capsid

2016 · Koen Vercauteren, Brad E Hoffman, et al. · Molecular Therapy · 114

A Broad RNA Virus Survey Reveals Both miRNA Dependence and Functional Sequestration

2016 · Troels K.H. Scheel, Joseph M. Luna, et al. · Cell Host & Microbe · 136

miRNA–target chimeras reveal miRNA 3′-end pairing as a major determinant of Argonaute target specificity

2015 · Michael J. Moore, Troels K. H. Scheel, et al. · Nature Communications · 324

Micropatterned coculture of primary human hepatocytes and supportive cells for the study of hepatotropic pathogens

2015 · Sandra March, Vyas Ramanan, et al. · Nature Protocols · 149

Mopping up miRNA: An integrated HBV transcript disrupts liver homeostasis by sequestering miR-122

2015 · Joseph M. Luna, Eleftherios Michailidis, et al. · Journal of Hepatology · 12

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus

2015 · Vyas Ramanan, Amir Shlomai, et al. · Scientific Reports · 304

Degradable hydrogels derived from PEG‐diacrylamide for hepatic tissue engineering

2015 · Kelly R. Stevens, Jordan S. Miller, et al. · Journal of Biomedical Materials Research Part A · 83

Hepatitis C virus infects rhesus macaque hepatocytes and simianized mice

2015 · Margaret A. Scull, Chao Shi, et al. · Hepatology · 25

Hepatitis C Virus RNA Functionally Sequesters miR-122

2015 · Joseph M. Luna, Troels K.H. Scheel, et al. · Cell · 332

Identification, Molecular Cloning, and Analysis of Full-Length Hepatitis C Virus Transmitted/Founder Genotypes 1, 3, and 4

2015 · Mark B. Stoddard, Hui Li, et al. · mBio · 18

Characterization of nonprimate hepacivirus and construction of a functional molecular clone

2015 · Troels K. H. Scheel, Amit Kapoor, et al. · Proceedings of the National Academy of Sciences · 100