Establishment of ALS model by artificial RNA-binding proteins related to ALS proteinopathies.
Description: The aim of this study was to generate a novel ALS model, knocked-in mouse of artificially designing cDNA harboring with the characteristics of the ALS causative genes causing ALS and introducing into the ROSA26 locus to examined their cytotoxicity in vivo.We were able to obtain a line of ALS artificial gene knock-in mice. Currently, we are planning to breed them. On the other hand, from the analysis of our established ALS model mouse (△NLS-FUS transgenic), we found that a wide variety of splicing changes were observed in the brain and spinal cord and also that semaphorin 3G, which is required for synaptic plasticity, was upregulated in this mouse.
Amount: ¥4,420,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Daisuke ITO
Institution: Keio University
Subfield: Neurology
Field: Medicine
Domain: Health Sciences
Funded outputs count: 7
Funder award ID: 18K07534
OpenAlex ID: G8119783783