Study on the role of the mechanical dynamics in the nucleus in cellular mechanical responses
Description: The nuclear deformation and strain caused by the mechanical forces transmitted from outside of the cell to the nucleus via cytoskeletons can affect the accessibility of mechanosensitive transcription factors and ultimately gene expression. Thus, understanding nuclear mechanical properties and deformation against mechanical stimuli is the key to revealing how nuclear mechanics controls the biochemical activity. In this study, we explored intranuclear mechanical properties and the mechanical relationship between the nucleus and cellular traction forces. As a result, we found a heterogenous distribution of mechanical properties in the nucleus, which is thought to be determined by intranuclear structure components other than DNA condensation states. Our results also suggest that the nucleus play an important role in intracellular distributions of actin cytoskeletons and traction forces.
Amount: ¥17,810,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (B)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: NAOYA SAKAMOTO
Institution: Tokyo Metropolitan University
Funded outputs count: 9
Funder award ID: 18H03521
OpenAlex ID: G8470778697