Control of Neural crest stem cell like MSC spheroids with DNA methylation
Description: Studies on human and animal models have demonstrated the complex molecular regulatory network between dental mesenchyme and epithelium governing tooth development. However, epigenetic regulation of tooth development has not been well investigated. The aim of this study was to elucidate the relationship between an epigenetic modifier and dental root development using Hdac3-CKOosx mice. We show that Hdac3 depletion in Osterix expressing dental pulp stem cells, odontoblasts, and cementocytes cause a progressive postnatal obstruction resulting in comparatively short roots and smaller root apices of the molar. We observed degeneration in the histological development of dentin and cementum structures. Dentin and cementum had shaky borders and disordered hematoxylin staining; in addition, the Hdac3-CKOosx had thin cementum compared to that in the wild-type mice. These findings show that Hdac3 is essential in dental mesenchymal cells and regulates the rate of root formation and development.
Amount: ¥15,600,000
Funding type: research
Funder scheme: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
Investigator: Kunimichi Niibe
Institution: Tohoku University
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 4
Funder award ID: 17KK0182
OpenAlex ID: G8774726446